Description of CS membrane coordination measurements:


1. Sample images were loaded into FIJI software.

2. Images were thresholded with 'Huang' auto-adjustment generating binary images.

3. Segmented line with 5 pixel width drawn across the original image following the membrane. Line positioning was adjusted to fit both membranes well. Line selections copy-pasted (Ctrl+Shift+E) to binary images.

4. Intensity profiles were measured on binary images (Ctrl+K) and data saved as .csv file for further processing in R (find R script located in this folder).




